What CBC parameters are affected by hemolysis?
It is thought that hemolysis is caused by damage to red blood cells, which can affect red blood cell count (RBC), mean corpuscular hemoglobin concentration (MCHC), and hematocrit (HCT), while the cell debris left from the broken red blood cells may also affect platelet count.
What lab values show hemolysis?
A standard workup for hemolysis includes lactate dehydrogenase (LDH), unconjugated bilirubin, and haptoglobin tests, as well as a reticulocyte count. Hemolysis is confirmed by increases in the reticulocyte count, LDH, and unconjugated bilirubin, along with decreased haptoglobin.
What analytes are affected by hemolysis?
Two analytes greatly impacted by hemolysis are potassium and lactate dehydrogenase, in which their concentrations in erythrocytes are more than 20 times and 150 times higher than it is in serum, respectively.
What tests are most affected by haemolysis?
But it’s not just potassium that hemolysis affects. These other tests are affected when hemolyzed specimens are submitted for testing: LDH, AST, ALT, phosphorous, magnesium, ammonia, RBC, hemoglobin and hematocrit. Not only these analytes, but virtually everything that could be tested.
Does hemolysis affect potassium?
Of all routine blood tests plasma/serum potassium measurement is one of the most sensitive to the effect of hemolysis because red-cell potassium concentration is so much higher than that of plasma (approximately 20 times higher); hemolysis causes a spuriously high plasma potassium concentration.
Is creatinine affected by hemolysis?
Specimen and Processing Considerations
On the other side, hemolysis may falsely decrease creatinine, alkaline phosphatase (ALP), potassium, packed cell volume (PCV), and mean corpuscular volume (MCV).
What labs are elevated in hemolysis?
Typical signs of in vivo hemolysis are an elevation of indirect bilirubin level and reticulocyte count, which is an indicator of marrow compensatory response. In in vitro hemolysis, the reticulocyte counts remaining normal. An elevation of LDH activity is typical of intravascular hemolysis.
How does hemolysis affect potassium levels?
Does hemolysis affect sodium level?
As previously reported, in vitro hemolysis is known to negatively interfere with sodium due to a diluting effect (13,14), as the intracellular concentration of sodium is significantly lower than the concentration in serum or plasma.
Is sodium level affected by hemolysis?
Are electrolytes affected by hemolysis?
Although a mild degree of hemolysis is considered to cause negligible changes in chemistry results, moderate to severe hemolysis has the potential to alter particularly electrolytes, minerals, and protein electrophoresis to a degree that may affect clinical decisions.
Is CPK affected by hemolysis?
Because the concentration of CPK is not significant in red blood cells, CPK levels are not affected by hemolysis. CPK is not affected by liver dysfunction, as are the other tested enzymes (eg, transaminases, aldolase, lactate dehydrogenase). High CPK levels represent leakage of the enzyme from the muscle cells only.
Is potassium affected by hemolysis?
How does hemolysis affect creatinine?
Hemolysis can cause falsely elevated creatine kinase (CK) values when spectrophotometric methods of measurement are used. This apparent increase in CK is due to the red blood cell enzyme adenylate kinase.
Does hemolysis affect blood glucose?
Hemolysis may falsely increase the following analytes: AST, alanine transaminase (ALT), LDH, total bilirubin, glucose, calcium, phosphorus, total protein, albumin, magnesium, amylase, lipase, creatine kinase (CK), iron, hemoglobin, and mean corpuscular hemoglobin concentration (MCHC).
Is CRP affected by hemolysis?
In the present study, hemolysis produced significant changes in CRP and Cp values.