What is FOXP3 a marker for?
FOXP3 is a specific marker for naturally occurring regulatory T cells (nTreg). FOXP3 expression is correlated with development and function of nTreg. Recent evidence suggests that, upon activation, human effector T (Teff) cells and type 1 regulatory T (Tr1) cells can express FOXP3, albeit transiently.
What activates FOXP3?
Foxp3 transcription is induced by T cell receptor (TCR) signaling and temporally persistent TCR signals activate Foxp3 transcription in self-reactive thymocytes (7, 8).
Do Jurkat cells express FOXP3?
FOXP3 is a transcription factor, belonging to the forkhead family (11), and it has been shown that FOXP3, overexpressed in Jurkat cells, can act as a repressor of transcription of the IL-2 promoter by competing with the binding of NF-AT (12) or by directly interacting with NF-AT or NF-κB (13).
What cell types express FOXP3?
It has been considered initially as the only specific marker for Treg. However, recent work has proposed that Foxp3 can be expressed by other types of lymphoid cells or myeloid cells and also by some non-hematopoietic cells such as epithelial cells.
What is FOXP3 test?
FOXP3 is a specific marker for adult T-cell leukemia/lymphoma (ATLL). Interpretation. This test does not include pathologist interpretation; only technical performance of the stain.
Is FOXP3 a surface marker?
FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers.
Is FOXP3 a cytokine?
Therefore, FoxP3 expression is an inherent component of equine T cell activation and is associated with a more immunosuppressive cytokine profile.
What does FOXP3 stand for?
forkhead box P3
FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells.
What are MT 2 cells?
The MT-2 cell line was derived from normal human cord leukocytes of a healthy donor by co-cultivation with leukemic cells from an adult T-cell leukemia (ATL) patient.
Is FOXP3 nuclear?
FOXP3 is known to act both as a nuclear transcriptional activator and repressor [35]. Therefore, one would expect that nuclear localization is essential for its transcriptional activity and that altered subcellular localization as we had observed in activated CD4+CD25− cells would affect its transcriptional activity.
Is FOXP3 a protein?
FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells.
Is FOXP3 intracellular?
88-8996-40). Cells in the lymphocyte gate were used for analysis. Foxp3 protein is difficult to stain since it is not very abundant, and it is an intracellular protein.
What are mt4 cells?
MT-4 cells were originally established by cocultivating cells from a 50-year-old, human T-cell lymphotropic virus type 1 (HTLV-1)-seropositive Japanese male with adult T-cell leukemia (ATL) with human cord blood lymphocytes (1).
What is CD25 a marker of?
CD25 is a marker for CD4+ thymocytes that prevent autoimmune diabetes in rats, but peripheral T cells with this function are found in both CD25+ and CD25− subpopulations. J Immunol. 2000;165:3105–10. [ PubMed] [Google Scholar]
What is FOXP3 Treg?
Transcriptional factor Foxp3 serves as a lineage specification factor of Treg cells. Paucity of Treg cells due to loss-of-function mutations of the Foxp3 gene is responsible for highly aggressive, fatal, systemic immune-mediated inflammatory lesions in mice and humans.
Is Foxp3 a surface marker?