What is APC Cy7?
APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm.
What is APC in flow cytometry?
APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
What is PE Cy7?
PE-Cyanine7 is a tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye degradation is common if exposed to photobleaching, fixation, and higher temperatures. This is a bright dye often used in flow cytometry for low-abundance antigens.
Can I use APC Cy7 and PE Cy7 together?
In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.
Can you use APC and PE Cy5 together?
PE-Cy5 (Ex-Max 496 nm/Em-Max 667 nm): is a tandem conjugate that combines phycoerythrin and a cyanine dye. Because of its broad absorption range and the fact that its emission spectrum is equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC.
What does APC stain for?
General notes. APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.
Can I use APC and PE Cy7 together?
Does PE overlap APC?
Furthermore, PE (and PE-Texas Red to an even greater extent) does overlap significantly with APC.
Which laser is PE?
PE is excitable by both the 488 nm laser and 561 nm laser, therefore PE and its tandem dyes appear here and on the 488 nm excitable fluorophores web page. As PE is maximally excited at 546 nm (compared to 496 nm using the 488 nm laser), where possible it is advisable to use the 561 nm laser to get the maximal signal.
Can you use PE Cy7 with APC Cy7?
Is APC brighter than PE?
It is also because APC is red-shifted compared to PE. Thus, the light source used to maximally excite APC will contain less energy than the one used to maximally excite PE. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
What Colour is APC?
Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields.
Can I use PE and PE Cy5 together?
Pe-‐CF594 spill over a lot from and to Pe, don’t use together. PeCy5 spill over a lot to APC (bad), Pe and PeCy7. Avoid these combinations if possible.
Can APC and APC Cy7 be used together?
Due to nearly identical excitation and emission properties, APC-Cy7 and APC-H7 cannot be used simultaneously. 633 nm 635 nm 640 nm 780/60 APC-H7 (Exmax650 nm/Emmax785 nm) is an APC-cyanine tandem fluorochrome which uses an analog of Cy7 and has similar spectral properties to APC-Cy7.
Does PE and APC overlap?
Third, APC emission at long wavelengths does not overlap significantly with PE emission.
Can you use PE Cy7 and APC Cy7?
Can you use APC and PE-Cy5 together?
How do you choose fluorochromes for flow cytometry?
Fluorochromes are selected based on their abilities to fluoresce with the wavelengths of light produced by the lasers. Therefore, if a flow cytometer has only one laser that produces only 488 nm light, then only fluorochromes that are excited by 488 nm light can be used.
What is fluorochromes in flow cytometry?
Fluorochromes used in flow cytometry are essentially those that can attach in some way to biologically significant molecules and are excitable by the lasers commonly found on commercial flow cytometers.
Is APC a good fluorophore?
Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.